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Alternaria gansuensis, a seed-borne fungus of standing milkvetch (Astragalus adsurgens), is the most common pathogen of this plant species and causes yellow stunt and root rot. Although plant resistance to this disease has been identified, a better understanding of the nature of this resistance will help improve and optimize its implementation in standing milkvetch. The effects of A. gansuensis on the physiology of standing milkvetch were assessed in a 4-week study comparing a resistant plant variety, Shanxi, and a susceptible variety, Ningxia. In the first week, there was an obvious decrease in photosynthesis (P) in inoculated plants, especially in the susceptible variety, but there were no changes in stomatal conductance (Sc). From the second week on, P and Sc decreased progressively, and significant stem lesions were observed concomitantly. Water use efficiency (WUE) increased slightly in the second week but then decreased significantly from the third week. Physiological changes observed for the resistant variety of standing milkvetch were less dramatic than those of the susceptible variety. Hyphae were observed around inoculation lesions of the plants. Culture filtrate (CF) of A. gansuensis induced changes in extracellular pH and conductivity, especially in the susceptible variety samples. Tissue integrity changes in the plants correlated with the decrease in P. Secondary metabolite compounds were extracted from the plants and 21 types of compounds were identified. The composition and proportion of secondary metabolites were markedly altered by the pathogen, and these differences may indicate potential mechanisms of disease resistance to A. gansuensis in standing milkvetch. 

The purpose of this study was to evaluate the reliability and validity of the raw accelerometry output from research-grade and consumer wearable devices compared to accelerations produced by a mechanical shaker table. Raw accelerometry data from a total of 40 devices (i.e., n = 10 ActiGraph wGT3X-BT, n = 10 Apple Watch Series 7, n = 10 Garmin Vivoactive 4S, and n = 10 Fitbit Sense) were compared to reference accelerations produced by an orbital shaker table at speeds ranging from 0.6 Hz (4.4 milligravity-mg) to 3.2 Hz (124.7mg). Two-way random effects absolute intraclass correlation coefficients (ICC) tested inter-device reliability. Pearson product moment, Lin’s concordance correlation coefficient (CCC), absolute error, mean bias, and equivalence testing were calculated to assess the validity between the raw estimates from the devices and the reference metric. Estimates from Apple, ActiGraph, Garmin, and Fitbit were reliable, with ICCs = 0.99, 0.97, 0.88, and 0.88, respectively. Estimates from ActiGraph, Apple, and Fitbit devices exhibited excellent concordance with the reference CCCs = 0.88, 0.83, and 0.85, respectively, while estimates from Garmin exhibited moderate concordance CCC = 0.59 based on the mean aggregation method. ActiGraph, Apple, and Fitbit produced similar absolute errors = 16.9mg, 21.6mg, and 22.0mg, respectively, while Garmin produced higher absolute error = 32.5mg compared to the reference. ActiGraph produced the lowest mean bias 0.0mg (95%CI = -40.0, 41.0). Equivalence testing revealed raw accelerometry data from all devices were not statistically significantly within the equivalence bounds of the shaker speed. Findings from this study provide evidence that raw accelerometry data from Apple, Garmin, and Fitbit devices can be used to reliably estimate movement; however, no estimates were statistically significantly equivalent to the reference. Future studies could explore device-agnostic and harmonization methods for estimating physical activity using the raw accelerometry signals from the consumer wearables studied herein. 

Abstract We describe and interpret deposits associated with the final Ubehebe Crater-forming, phreatomagmatic explosive phase of the multivent, monogenetic Ubehebe volcanic center. Ubehebe volcano is located in Death Valley, California, USA. Pyroclastic deposits occur in four main facies: (1) lapilli- and blockdominated beds, (2) thinly bedded lapilli tuff, (3) laminated and cross-laminated ash, and (4) massive lapilli ash/tuff. Lapilli- and block-dominated beds are found mostly within several hundred meters of the crater and transition outward into discontinuous lenses of lapilli and blocks; they are interpreted to have been deposited by ballistic processes associated with crater-forming explosions. Thinly bedded lapilli tuff is found mainly within several hundred meters, and laminated and cross-laminated ash extends at least 9 km from the crater center. Dune forms are common within ~2 km of the crater center, while finer-grained, distal deposits tend to exhibit planar lamination. These two facies (thinly bedded lapilli tuff and laminated and cross-laminated ash) are interpreted to record multiple pyroclastic surges (dilute pyroclastic currents). Repeated couplets of coarse layers overlain by finer-grained, laminated horizons suggest that many or most of the surges were transient, likely recording individual explosions, and they traveled over complex topography in some areas. These two factors complicate the application of classical sediment-transport theory to quantify surge properties. However, dune-form data provide possible constraints on the relationships between suspended load sedimentation and bed-load transport that are consistent using two independent approaches. Massive lapilli ash/tuff beds occur in drainages below steep slopes and can extend up to ~1 km onto adjacent valley floors beneath large catchments. Although they are massive in texture, their grain-size characteristics are shared with laminated and cross-laminated ash facies, with which they are locally interbedded. These are interpreted to record concentrated granular flows sourced by remobilized pyroclastic surge deposits, either during surge transport or shortly after, while the surge deposits retained their elevated initial pore-gas pressures. Although similar surge-derived concentrated flows have been described elsewhere (e.g., Mount St. Helens, Washington, USA, and Soufriére Hills, Montserrat, West Indies), to our knowledge Ubehebe is the first case where such processes have been identified at a maar volcano. These concentrated flows followed paths that were independent of the pyroclastic surges and represent a potential hazard at similar maar volcanoes in areas with complex terrain. 

Aspergillus flavus is an agriculturally important fungus that causes ear rot of maize and produces aflatoxins, of which B 1 is the most carcinogenic naturally-produced compound. In the US, the management of aflatoxins includes the deployment of biological control agents that comprise two nonaflatoxigenic A . flavus strains, either Afla-Guard (member of lineage IB) or AF36 (lineage IC). We used genotyping-by-sequencing to examine the influence of both biocontrol agents on native populations of A . flavus in cornfields in Texas, North Carolina, Arkansas, and Indiana. This study examined up to 27,529 single-nucleotide polymorphisms (SNPs) in a total of 815 A . flavus isolates, and 353 genome-wide haplotypes sampled before biocontrol application, three months after biocontrol application, and up to three years after initial application. Here, we report that the two distinct A . flavus evolutionary lineages IB and IC differ significantly in their frequency distributions across states. We provide evidence of increased unidirectional gene flow from lineage IB into IC, inferred to be due to the applied Afla-Guard biocontrol strain. Genetic exchange and recombination of biocontrol strains with native strains was detected in as little as three months after biocontrol application and up to one and three years later. There was limited inter-lineage migration in the untreated fields. These findings suggest that biocontrol products that include strains from lineage IB offer the greatest potential for sustained reductions in aflatoxin levels over several years. This knowledge has important implications for developing new biocontrol strategies. 

Abstract Hazard assessments in monogenetic volcanic fields require estimates of the runout of pyroclastic surges that result from phreatomagmatic explosive activity. Previous assessments used runout distances of 1–4 km, with large cases up to 6 km. Surge deposits at Ubehebe Crater (∼2100 y.b.p., Death Valley, California) have been traced ∼9 km from the crater center, and likely originally extended 1–3 km farther. There is no evidence that the Ubehebe Crater activity was unusually energetic; rather, its distal deposits are better preserved than those at most maar volcanoes because of its young age and the arid environment. Numerical simulations illustrate how low temperatures facilitate long runout of phreatomagmatic surges due to reduced expansion of entrained air compared to hot surges, allowing cool surges to retain higher densities than ambient air. We suggest that hazard assessments for volcanic fields with phreatomagmatic, maar‐forming eruptions should consider runout distances in the range of 10–15 km. 

Abstract Preserved rocks in the Jurassic Ferrar Large Igneous Province consist mainly of intrusions, and extrusive rocks, the topic of this chapter, comprise the remaining small component. They crop out in a limited number of areas in the Transantarctic Mountains and southeastern Australia. They consist of thick sequences of lavas and sporadic occurrences of volcaniclastic rocks. The latter occur mainly beneath the lavas and represent the initial eruptive activity, but also are present within the lava sequence. The majority are basaltic phreatomagmatic deposits and in at least two locations form immense phreatocauldrons filled with structureless tuff breccias and lapilli tuffs with thicknesses of as much as 400 m. Stratified sequences of tuff breccias, lapilli tuffs and tuffs are up to 200 m thick. Thin tuff beds are sparsely distributed in the lava sequences. Lava successions are mainly 400–500 m thick, and comprise individual lavas ranging from 1 to 230 m thick, although most are in the range of 10–100 m. Well-defined colonnade and entablature are seldom displayed. Lava sequences were confined topographically and locally ponded. Water played a prominent role in eruptive activity, as exhibited by phreatomagmatism, hyaloclastites, pillow lava and quenching of lavas. Vents for lavas have yet to be identified.